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. 2003 Dec;41(12):5414-8.
doi: 10.1128/JCM.41.12.5414-5418.2003.

Use of an immunoglobulin G avidity assay based on recombinant antigens for diagnosis of primary Toxoplasma gondii infection during pregnancy

Elisa Beghetto  1 , Wilma Buffolano, Andrea Spadoni, Mariassunta Del Pezzo, Manlio Di Cristina, Olga Minenkova, Eskild Petersen, Franco Felici, Nicola Gargano
Affiliations

Use of an immunoglobulin G avidity assay based on recombinant antigens for diagnosis of primary Toxoplasma gondii infection during pregnancy

Elisa Beghetto et al. J Clin Microbiol. 2003 Dec.

Abstract

The objective of this work was to develop an antibody-specific immunoglobulin G (IgG) avidity assay to discriminate between acute and latent phases of Toxoplasma gondii infection by using recombinant antigens. One hundred twenty-one serum samples from women who developed IgG antibodies against Toxoplasma during pregnancy were used. The IgG avidities of antibodies directed against epitopes carried by fragments of GRA3, GRA7, MIC3, and SAG1 antigens were measured by performing parallel enzyme immunoassays. The avidity index for Toxoplasma-specific antibodies against a homogeneous mixture of recombinant GRA3, GRA7, MIC3, and SAG1 antigens correlated closely with the IgG avidity of antibodies against lysed whole-cell T. gondii antigen. The avidity assay performed with the recombinant MIC3 antigen highlighted the presence of avidity low-antibodies IgG exclusively in sera collected within 2 months after primary infection. The presence of T. gondii-specific, low-avidity IgG antibodies against recombinant MIC3 antigen can be used to determine the point of infection with T. gondii within a 2-month time frame after infection.

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Figures

FIG. 1.
FIG. 1.
IgG avidity assay with MIC3 antigen. (A) Toxoplasma-specific IgG avidity index for lysed whole-cell T. gondii antigen (Vidas system; bioMérieux). (B) Toxoplasma-specific IgG avidity index for recombinant GST-MIC3 and elution buffer with 8 M urea.
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