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. 2003 Dec;41(12):5572-6.
doi: 10.1128/JCM.41.12.5572-5576.2003.

Molecular analysis of Cryptococcus neoformans mitochondrial cytochrome b gene sequences

Swarajit Kumar Biswas  1 , Li Wang, Koji Yokoyama, Kazuko Nishimura
Affiliations

Molecular analysis of Cryptococcus neoformans mitochondrial cytochrome b gene sequences

Swarajit Kumar Biswas et al. J Clin Microbiol. 2003 Dec.

Abstract

Mitochondrial cytochrome b genes (cyt b) of 40 strains of Cryptococcus neoformans were partially sequenced to determine the genetic relations. With the exception of the type strain of C. neoformans var. neoformans, all strains contained introns in their sequences. Analysis of 386 bp of coding sequence from each strain under investigation revealed a total of 27 (6.99%) variable nucleotide sites and categorized isolates of C. neoformans into nine cyt b types. C. neoformans var. gattii included cyt b types I to V, and C. neoformans var. neoformans comprised types VI to IX. cyt b types were correlated with serotypes. All strains with cyt b types I, IV, and V were serotype B. All other strains except IFM 5878 (serotype B) with cyt b types II and III were serotype C. Serotype D strains had cyt b types VI and IX, and serotype A strains were cyt b type VIII. Of four serotype AD strains, one was cyt b type VII and the remaining three were type VIII. The phylogenetic tree based on deduced amino acid sequences divided the strains only into C. neoformans var. neoformans and C. neoformans var. gattii. These results indicate that cyt b sequences are effective for DNA typing as well as phylogenetic analysis of C. neoformans.

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Figures

FIG. 1.
FIG. 1.
Comparison of coding sequences of the mt cyt b genes of various C. neoformans isolates. Dots, nucleotides that are identical to those of C. neoformans cyt b type I; arrows, positions of introns. Cyt b types are in parentheses.
FIG. 2.
FIG. 2.
Comparison of the deduced amino acid sequences encoded by the cyt b genes of various C. neoformans isolates. Dots, amino acids that are identical to those of C. neoformans cyt b type I; arrows, inserted positions of introns. cyt b types are in parentheses.
FIG. 3.
FIG. 3.
UPGMA-based trees showing the relations of various C. neoformans isolates generated from nucleotide sequences of the cyt b gene (exon) (a) and deduced amino acid sequences (b). Bar, number of nucleotide and amino acid substitutions per nucleotide site and amino acid site.
See this image and copyright information in PMC

References

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