Histochemical characterization of masseter muscle fibres in a biopsy study of normal young women

Abstract

Muscle fibres from biopsies of the anterior part of the masseter muscle (pars superficialis) were histochemically characterized in 13 healthy female students. They were 21-28 yr old with a full complement of teeth, and normal facial and occlusal relations. Before surgery, normal masseter muscle function was demonstrated by bite-force measurements and recordings of electromyographic activity. After staining for myosin ATPase activity, the relative mean areas of muscle fibres were: type I 82.9%, type IM 9.5% and type II 8.3%. The intraindividual (18-155% of mean) and interindividual (0-216% of mean) variation of the fibre size was large. The type I fibres had the largest diameter (10-80 microns, mean: 39 microns), the type II fibres (6-47 microns, mean: 21 microns) and the IM fibres (8-54 microns, mean: 28 microns) the smallest. The biopsy technique and the histochemical characterization will be suitable for reference in women with functional changes or diseases of the masseter muscle.