Seed production of Arabidopsis thaliana under hypobaric conditions

Abstract

In the present study, we examined carbohydrate contents of Arabidopsis thaliana seeds during seed development under hypobaric conditions in order to characterize the mechanism of low pressure-induced seed damage, and to determine critical pressures for seed development under low total and/or low oxygen partial pressures. We analyzed contents of starch, sucrose, glucose, and fructose in seeds at different developmental stages at 101 kPa total pressure with 21 kPa O2 partial pressure (control conditions), and at various low pressure conditions (23 kPa Ptot/21 kPa pO2, 101 kPa Ptot/2 kPa pO2, 53 kPa Ptot/2 kPa pO2, 23 kPa Ptot/2 kPa pO2, 12 kPa Ptot/10 kPa pO2, and 12 kPa Ptot/2 kPa pO2). Our results indicate that maintaining an adequate oxygen partial pressure inside the siliques is necessary for seed production under hypobaric conditions.