Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2004 Feb 19;23(7):1457-68.
doi: 10.1038/sj.onc.1207265.

Characterization of human exonuclease 1 in complex with mismatch repair proteins, subcellular localization and association with PCNA

Finn Cilius Nielsen  1 , Anne Charlotte Jäger, Anne Lützen, Jens R Bundgaard, Lene Juel Rasmussen
Affiliations

Characterization of human exonuclease 1 in complex with mismatch repair proteins, subcellular localization and association with PCNA

Finn Cilius Nielsen et al. Oncogene. .

Abstract

Human exonuclease 1 (hEXO1) has been implicated in DNA mismatch repair (MMR), replication, and recombination, but the nature of its interaction with these cellular processes is still ambiguous. We show that hEXO1 colocalizes with proliferating cell nuclear antigen (PCNA) at DNA replication sites and that the C-terminal region of hEXO1 is sufficient for this localization. We also show that both hMLH1-hPMS2 (MutLalpha) and hMLH1-hEXO1 complexes are formed in a reaction mixture containing all three proteins. Moreover, hEXO1 5' double-stranded exonuclease activity on a homoduplex substrate but not on a substrate containing a G/T mismatch was inhibited by complex formation with hMSH2-hMSH6 (MutSalpha) or MutLalpha. Taken together, the results support a model in which hEXO1 plays a role in events at the replication sites as well as a functional role in the MMR and/or recombination processes.

PubMed Disclaimer

Publication types