Review
doi: 10.1016/j.pep.2003.08.023.
In vitro protein refolding by chromatographic procedures
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- PMID: 14680955
- DOI: 10.1016/j.pep.2003.08.023
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Review
In vitro protein refolding by chromatographic procedures
Protein Expr Purif.
2004 Jan.
Abstract
In vitro protein refolding is still a bottleneck in both structural biology and in the development of new biopharmaceuticals, especially for commercially important polypeptides that are overexpressed in Escherichia coli. This review focuses on protein refolding methods based on column procedures because recent advances in chromatographic refolding have shown promising results.
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