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Review
. 2004 Jan;33(1):1-10.
doi: 10.1016/j.pep.2003.08.023.

In vitro protein refolding by chromatographic procedures

Ming Li  1 , Zhi-Guo Su, Jan-Christer Janson
Affiliations
Review

In vitro protein refolding by chromatographic procedures

Ming Li et al. Protein Expr Purif. 2004 Jan.

Abstract

In vitro protein refolding is still a bottleneck in both structural biology and in the development of new biopharmaceuticals, especially for commercially important polypeptides that are overexpressed in Escherichia coli. This review focuses on protein refolding methods based on column procedures because recent advances in chromatographic refolding have shown promising results.

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