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. 2004 Jan;48(1):329-32.
doi: 10.1128/AAC.48.1.329-332.2004.

blaVIM-7, an evolutionarily distinct metallo-beta-lactamase gene in a Pseudomonas aeruginosa isolate from the United States

Mark A Toleman  1 , Kenneth Rolston, Ronald N Jones, Timothy R Walsh
Affiliations

blaVIM-7, an evolutionarily distinct metallo-beta-lactamase gene in a Pseudomonas aeruginosa isolate from the United States

Mark A Toleman et al. Antimicrob Agents Chemother. 2004 Jan.

Abstract

As part of the CANCER Antimicrobial Surveillance Program in North America, a Pseudomonas aeruginosa isolate, strain 07-406, was shown to possess a metallo-beta-lactamase, designated VIM-7. bla(VIM-7) is located on a 24-kb plasmid which can be readily transferred into Enterobacteriaceae and other pseudomonads. This is the first report of a mobile metallo-beta-lactamase gene, bla(VIM-7), being detected within the United States.

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Figures

FIG. 1.
FIG. 1.
Nucleotide sequence of the 1,546-bp insert of recombinant plasmid pMATVIM-7 containing the blaVIM-7 coding sequence. The start codon of blaVIM-7 is indicated with a horizontal arrow, and the stop codon is indicated with an asterisk. The deduced amino acid sequence is reported below the gene sequence. The conserved core and inverse core sites are enclosed in boxes, and the attI1 site is highlighted in italicized capital letters.
FIG. 2.
FIG. 2.
Phylogenetic tree showing the relatedness of VIM-7 to other mobile metallo-β-lactamases. The phylogenetic tree is based on a CLUSTAL W alignment of a metallo-β-lactamase proteins generated by using the PAM250 MATRIX.
FIG. 3.
FIG. 3.
Genetic context of VIM-7 and VIM metallo-β-lactamases. A schematic map of the recombinant plasmid PMATVIM-7 carrying blaVIM-7 (accession no. AJ536835 [A]) is compared with examples of structures of blaVIM-2-containing class 1 integrons isolated in France (accession no. AF191564 [B]) and Korea (accession no. AF369871 [C] and accession no. AY030343 [D]) and with the blaVIM-1 integron isolated from Italy (accession AJ278514 [E]). Hatched rectangles indicate 5′ and 3′ CSs, black circles indicate 59-bp elements, and open ellipses indicate attI1 sites. Open reading frames of the various resistance genes are boxed, with an arrow indicating the direction of transcription. The solid line in pMATVIM-7 indicates the clones' insert from P. aeruginosa 07-406, and dotted lines indicate the cloning vector pK18.
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