Effects of cortisol on food intake, growth, and forebrain neuropeptide Y and corticotropin-releasing factor gene expression in goldfish

Abstract

Although elevated plasma cortisol levels and a reduction in food intake are common features of the response to stress in fish, the potential role of cortisol in the regulation of food intake in these animals is poorly understood. In this study, goldfish (Carassius auratus) were fed ad libitum for 21 days diets prepared to contain 0 (Control), 50 (Low) or 500 (High) microg cortisol/g of food. While feeding remained unchanged in controls and in fish fed the High cortisol diet, daily food intake gradually increased in the Low cortisol diet group and was significantly elevated between days 9 and 21. At the end of the feeding trial, specific growth rate was lowest in fish fed the High cortisol diet, intermediate in those fed the Low cortisol diet, and highest in the controls. Feed conversion efficiency, on the other hand, was significantly reduced in both groups of fish fed the cortisol diets. After 3 weeks on the diets and relative to controls, the Low cortisol diet group was characterized by a 34% increase in neuropeptide Y (NPY) and a 22% decrease in corticotropin-releasing factor (CRF) mRNA levels in the telencephalon-preoptic brain region. In contrast, the High cortisol diet group was characterized by a 46% decrease in CRF mRNA levels and no significant change in NPY gene expression. In a separate experiment, intraperitoneal implants of cortisol (150 and 300 microg cortisol/g body weight) elicited a dose-dependent increase in NPY and decrease in CRF mRNA levels in the telencephalon-preoptic region at 72 h post-treatment. These results show that while moderate increases in plasma cortisol can stimulate food intake slowly over days, larger catabolic doses of glucocorticoids may mask the appetite-stimulatory effects of cortisol. Therefore, excess cortisol in goldfish can be associated with poor growth despite normal food intake. Furthermore, our results indicate that forebrain NPY and CRF may play a role in mediating the effects of cortisol on food intake in goldfish.