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. 1992 Sep-Oct;26(5):999-1010.

[A method of isolation and properties of DNA-dependent DNA-polymerase epsilon from human placenta]

[Article in Russian]
D Iu Mozzherin, A M Atrazhev, M K Kukhanova
  • PMID: 1470182

[A method of isolation and properties of DNA-dependent DNA-polymerase epsilon from human placenta]

[Article in Russian]
D Iu Mozzherin et al. Mol Biol (Mosk). 1992 Sep-Oct.

Abstract

DNA polymerase epsilon was purified to near homogeneity from human placenta. The enzyme has one subunit (170 kDa, sedimentation coefficient 8.2S), intrinsic 3'-5'-exonuclease activity, it is independent on PCNA and high processivity on poly(dA)-oligo(dT) template-primer without PCNA. It was shown, that the enzyme incorporates 3'-amino-2',3'-dideoxythymidine 5'-triphosphate in DNA, after that synthesis is stopped. Simultaneously DNA polymerase alpha was purified.

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