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Comparative Study
. 2003 Dec;165(4):1823-30.
doi: 10.1093/genetics/165.4.1823.

Characterization of a male-predominant antisense transcript underexpressed in hybrids of Drosophila pseudoobscura and D. persimilis

Affiliations
Comparative Study

Characterization of a male-predominant antisense transcript underexpressed in hybrids of Drosophila pseudoobscura and D. persimilis

Mohamed A F Noor et al. Genetics. 2003 Dec.

Abstract

Characterizing genes that are misregulated in hybrids may elucidate the genetic basis of hybrid sterility or other hybrid dysfunctions that contribute to speciation. Previously, a small segment of a male-predominant transcript that is underexpressed in adult male hybrids of Drosophila pseudoobscura and D. persimilis relative to pure species was identified in a differential display screen. Here, we obtained the full sequence of this 1330-bp transcript and determined that it is an antisense message with high sequence similarity to the D. melanogaster TRAP100 gene, part of the Mediator protein complex that regulates transcriptional initiation during development. Both the sense and the antisense messages are transcribed in D. pseudoobscura, but only the sense message (TRAP100) is transcribed in D. melanogaster complex species. Unlike the antisense message, the sense message is transcribed similarly in D. pseudoobscura males and females and in hybrids of D. pseudoobscura and D. persimilis. The high sequence similarity between distantly related species suggests that the sense message is functionally constrained within the genus. We speculate that the antisense transcript may have evolved a role in male-specific post-transcriptional regulation of TRAP100 in the D. pseudoobscura lineage and that its underexpression in sterile hybrid males may cause an overproduction of TRAP100 protein, possibly yielding deleterious effects.

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References

    1. Science. 1998 Nov 20;282(5393):1501-4 - PubMed
    1. Philos Trans R Soc Lond B Biol Sci. 1998 Feb 28;353(1366):287-305 - PubMed
    1. Genetics. 1999 Nov;153(3):1385-94 - PubMed
    1. Dev Biol. 1999 Dec 15;216(2):671-80 - PubMed
    1. J Hered. 2000 Mar-Apr;91(2):99-103 - PubMed

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