Chalone inhibition of granulocyte colony growth in agar: kinetic quantitation by capillary tube scanning

Abstract

Mouse bone marrow cells were seeded into capillary tubes containing agar with colony stimulating factor. The development of myelomonocytic clusters and colonies was followed by daily tube scanning using their light scattering properties. Three kinetic scanning parameters were determined and the significance of different threshold settings was evaluated; viz. the number of signals, the mean signal height and the signal integrals. The inhibitory effect of two extracts with known granulocyte chalone activity which had been prepared from human peripheral leukocytes and rat bone marrow cells, was followed with the scanning method. A continuous reduction of clusters and colony formation and their growth throughout the incubation period was observed which suggested a sustained retardation of proliferation of both the stem cells committed for myelomonopoiesis and their progeny.