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. 2004 Jan;42(1):359-61.
doi: 10.1128/JCM.42.1.359-361.2004.

Development of an immunochromatographic test with recombinant EMA-2 for the rapid detection of antibodies against Babesia equi in horses

Xiaohong Huang  1 , Xuenan Xuan, Longshan Xu, Shoufa Zhang, Naoaki Yokoyama, Naoyoshi Suzuki, Ikuo Igarashi
Affiliations

Development of an immunochromatographic test with recombinant EMA-2 for the rapid detection of antibodies against Babesia equi in horses

Xiaohong Huang et al. J Clin Microbiol. 2004 Jan.

Abstract

An immunochromatographic test (BeICT) for the rapid detection of antibodies against Babesia equi was developed. It clearly differentiated B. equi-infected horses from B. caballi-infected and uninfected horses. The agreement with enzyme-linked immunosorbent assay results was 96.7% in the detection of field sera. The results suggest that BeICT is rapid, simple, reliable, and suitable for use to detect B. equi infection in the field.

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Figures

FIG. 1.
FIG. 1.
Examples of BeICT strips before (lane 1) and after (lanes 2 and 3) testing. Symbols: +, positive result; −, negative result.
FIG. 2.
FIG. 2.
Specific antibody responses in sequential sera from two horses (E3 and E4) experimentally infected with B. equi detected by ELISA and BeICT, respectively. A, IgG antibody titers examined by ELISA; B, antibody responses examined by BeICT.
See this image and copyright information in PMC

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