Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2004 Jan;42(1):449-52.
doi: 10.1128/JCM.42.1.449-452.2004.

Serodiagnosis of chronic and acute Chagas' disease with Trypanosoma cruzi recombinant proteins: results of a collaborative study in six Latin American countries

Eufrosina S Umezawa  1 , Alejandro O Luquetti, Gabriela Levitus, Carlos Ponce, Elisa Ponce, Diana Henriquez, Susana Revollo, Bertha Espinoza, Octavio Sousa, Baldip Khan, José Franco da Silveira
Affiliations

Serodiagnosis of chronic and acute Chagas' disease with Trypanosoma cruzi recombinant proteins: results of a collaborative study in six Latin American countries

Eufrosina S Umezawa et al. J Clin Microbiol. 2004 Jan.

Abstract

An enzyme-linked immunosorbent assay to diagnose Chagas' disease by a serological test was performed with Trypanosoma cruzi recombinant antigens (JL8, MAP, and TcPo). High sensitivity (99.4%) and specificity (99.3%) were obtained when JL8 was combined with MAP (JM) and tested with 150 serum samples from chagasic and 142 nonchagasic individuals. Moreover, JM also diagnosed 84.2% of patients in the acute phase of T. cruzi infection.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
(A) Reactivity data of T. cruzi recombinant antigens MAP, JL8, and TcPo individually or in various combinations of two or three proteins (JM, MT, and MJT) with sera from 11 Brazilian patients with well-defined chronic-phase Chagas' disease. (B) Reactivity data of recombinant mixtures JM, MT, MJT, and BHF with 19 acute-phase sera. The sensitivities of the different antigens or tests are shown at the bottom of the figure. EAE-ELISA data are shown in panels A and B. For each antigen, the average is indicated by the short horizontal line, and the arrow indicates the cutoff value.
FIG. 2.
FIG. 2.
Reactivity data of recombinant antigens JM with 150 serum samples from patients infected with T. cruzi from regions of Bolivia (Bol), Brazil (Br), Honduras (Ho), Mexico and Venezuela (Me/Ve), and Panama (Pa) where Chagas' disease is endemic. For each country, the average is indicated by the short horizontal line, and the arrow indicates the cutoff value.
See this image and copyright information in PMC

References

    1. Almeida, I. C., D. T. Covas, L. M. T. Soussumi, and L. R. Travassos. 1997. A highly sensitive chemiluminescence enzyme-linked immunosorbent assay for diagnosis of active Trypanosoma cruzi infection. Transfusion 37:850-857. - PubMed
    1. Breniere, S. F., N. Yaksic, N. Telleria, M. F. Bosseno, F. Noireau, P. Wincker, and D. Sanchez. 1997. Immune response to Trypanosoma cruzi shed acute phase antigen in children from an endemic area for Chagas' disease in Bolívia. Mem. Inst. Oswaldo Cruz 92:503-507. - PubMed
    1. Camargo, M. E., and V. Amato-Neto. 1974. Anti-Trypanosoma cruzi IgM antibodies as serological evidence of recent infection. Rev. Inst. Med. Trop. São Paulo 16:200-202. - PubMed
    1. Carvalho, M. R., M. A. Krieger, W. Oeleman, M. A. Shikanai-Yasuda, A. W. Ferreira, J. B. Pereira, A. Saez-Alquezar, D. F. Dorlhiac-Llacer, D. F. Chamone, and S. Goldenberg. 1993. Chagas' disease diagnosis: evaluation of several tests in blood bank screening. Transfusion 33:830-834. - PubMed
    1. Feliciangeli, M. D., D. Campbell-Lendrum, C. Martinez, D. Gonzalez, P. Coleman, and C. Davies. 2003. Chagas' disease control in Venezuela: lessons for the Andean region and beyond. Trends Parasitol. 19:44-49. - PubMed

Publication types