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. 2004 Jan;42(1):474-7.
doi: 10.1128/JCM.42.1.474-477.2004.

Transfer of a Mycobacterium tuberculosis genotyping method, Spoligotyping, from a reverse line-blot hybridization, membrane-based assay to the Luminex multianalyte profiling system

Lauren S Cowan  1 , Lois Diem, Mary Catherine Brake, Jack T Crawford
Affiliations

Transfer of a Mycobacterium tuberculosis genotyping method, Spoligotyping, from a reverse line-blot hybridization, membrane-based assay to the Luminex multianalyte profiling system

Lauren S Cowan et al. J Clin Microbiol. 2004 Jan.

Abstract

Spoligotyping using Luminex technology was shown to be a highly reproducible method suitable for high-throughput analysis. Spoligotyping of 48 isolates using the traditional membrane-based assay and the Luminex assay yielded concordant results for all isolates. The Luminex platform provides greater flexibility and cost effectiveness than the membrane-based assay.

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Figures

FIG. 1.
FIG. 1.
Hybridization patterns for three isolates obtained using the original, membrane-based assay. Lane 1, isolate 1, M. bovis; lane 2, isolate 2, M. tuberculosis Beijing family; and lane 3, isolate 3, M. tuberculosis Latino-American and Mediterranean family (1).
FIG. 2.
FIG. 2.
Comparison of Luminex-generated spoligotype results with traditional, membrane-based results for 48 clinical isolates. Using the results from the membrane-based assay, spacers in each isolate were identified as positive or negative in each strain. For each spacer, the average median divided by the background median was calculated for positive spacers (•) and for negative spacers (▪).
See this image and copyright information in PMC

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