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. 2004 Feb;10(2):203-7.
doi: 10.1038/nm981. Epub 2004 Jan 11.

Two-photon fluorescence correlation microscopy reveals the two-phase nature of transport in tumors

George Alexandrakis  1 , Edward B Brown, Ricky T Tong, Trevor D McKee, Robert B Campbell, Yves Boucher, Rakesh K Jain
Affiliations
Free article

Two-photon fluorescence correlation microscopy reveals the two-phase nature of transport in tumors

George Alexandrakis et al. Nat Med. 2004 Feb.
Free article

Abstract

Transport parameters determine the access of drugs to tumors. However, technical difficulties preclude the measurement of these parameters deep inside living tissues. To this end, we adapted and further optimized two-photon fluorescence correlation microscopy (TPFCM) for in vivo measurement of transport parameters in tumors. TPFCM extends the detectable range of diffusion coefficients in tumors by one order of magnitude, and reveals both a fast and a slow component of diffusion. The ratio of these two components depends on molecular size and can be altered in vivo with hyaluronidase and collagenase. These studies indicate that TPFCM is a promising tool to dissect the barriers to drug delivery in tumors.

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