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. 2004 Jan 16;303(5656):363-6.
doi: 10.1126/science.1090908.

Guar seed beta-mannan synthase is a member of the cellulose synthase super gene family

Kanwarpal S Dhugga  1 , Roberto Barreiro, Brad Whitten, Kevin Stecca, Jan Hazebroek, Gursharn S Randhawa, Maureen Dolan, Anthony J Kinney, Dwight Tomes, Scott Nichols, Paul Anderson
Affiliations

Guar seed beta-mannan synthase is a member of the cellulose synthase super gene family

Kanwarpal S Dhugga et al. Science. .

Abstract

Genes for the enzymes that make plant cell wall hemicellulosic polysaccharides remain to be identified. We report here the isolation of a complementary DNA (cDNA) clone encoding one such enzyme, mannan synthase (ManS), that makes the beta-1, 4-mannan backbone of galactomannan, a hemicellulosic storage polysaccharide in guar seed endosperm walls. The soybean somatic embryos expressing ManS cDNA contained high levels of ManS activities that localized to Golgi. Phylogenetically, ManS is closest to group A of the cellulose synthase-like (Csl) sequences from Arabidopsis and rice. Our results provide the biochemical proof for the involvement of the Csl genes in beta-glycan formation in plants.

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