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. 2003 Dec;67(12):2674-7.
doi: 10.1271/bbb.67.2674.

A transient RNA interference assay system using Arabidopsis protoplasts

Chung-Il An  1 , Aki Sawada, Ei-ichiro Fukusaki, Akio Kobayashi
Affiliations
Free article

A transient RNA interference assay system using Arabidopsis protoplasts

Chung-Il An et al. Biosci Biotechnol Biochem. 2003 Dec.
Free article

Abstract

Double-stranded RNA (dsRNA) induces sequence-specific gene silencing in eukaryotes through a process known as RNA interference (RNAi). RNAi is now used as a powerful tool for functional genomics in many eukaryotes, including plants. We herein report a dsRNA-mediated transient RNAi assay system using protoplasts from Arabidopsis mesophyll cells and suspension-cultured cells (cell line T87). Introduction of dsRNA into protoplasts led to marked silencing of target transgenes. Our assay system would provide a convenient and efficient way to induce RNAi in protoplasts of the model plant Arabidopsis thaliana.

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