[Distribution of type IV collagen, laminin and fibronectin during naris formation in the chick embryo]

Abstract

The presence and distribution of extracellular matrix (ECM) macromolecules were analyzed in the facial primordia of chick embryos from stages (22-28) of development corresponding to naris formation. Frozen sections through the nasal placode, medial nasal processes and lateral nasal processes were prepared for indirect immunofluorescence employing a biotin-avidin DCS system using monoclonal antibodies against type IV collagen, laminin and fibronectin. The results demonstrated that ECM played a very important role in regulating migration, adhesion and anchorage of cells during morphogenesis. Strong fluorescence of basal lamina was present in the areas of rapid ingrowth of the nasal placode. It was suggested that ECM has very important guiding and regulating role during naris formation in chick embryo.