Phosphate efflux from jejunal enterocytes of the rat: effect of phosphate concentration gradient and pH

Abstract

The effect of variation in the intracellular and extracellular phosphate concentration on the Pi efflux across the basolateral membrane of pre-loaded enterocytes has been examined. Efflux rate constants for Pi fell from 0.89 h-1 to 0.68 h-1 as the extracellular Pi concentration was increased from 0.5 mM to 5 mM. As the intracellular Pi concentration was raised from 0.5 to 3 mM the rate constant dropped from 0.95 h-1 to 0.77 h-1. The findings are indicative of the presence of Pi-specific transporter at the basolateral membrane. The efflux rate constant of Pi at pH 7.1 was higher than that at pH 7.4 suggesting that the Pi flux across the basolateral membrane of enterocytes follows a similar pattern towards pH changes as do fluxes across the brush-border membrane.