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. 2004 Jan 22;20(2):271-3.
doi: 10.1093/bioinformatics/btg401.

ROSO: optimizing oligonucleotide probes for microarrays

Nancie Reymond  1 , Hubert Charles, Laurent Duret, Federica Calevro, Guillaume Beslon, Jean-Michel Fayard
Affiliations

ROSO: optimizing oligonucleotide probes for microarrays

Nancie Reymond et al. Bioinformatics. .

Abstract

ROSO is software to design optimal oligonucleotide probe sets for microarrays. Selected probes show no significant cross-hybridization, no stable secondary structures and their Tm are chosen to minimize the Tm variability of the probe set.

Availability: The program is available on the internet. Sources are freely available, for non-profit use, on request to the authors.

Supplementary information: http://pbil.univ-lyon1.fr/roso

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