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. 1977 Mar 2;2(4):321-31.
doi: 10.1007/BF00537502.

Indication of the metarhodopsin I-II transition by absorption-changes of Eriochromblack T

H M Emrich, R Reich

Indication of the metarhodopsin I-II transition by absorption-changes of Eriochromblack T

H M Emrich et al. Biophys Struct Mech. .

Abstract

The dye eriochromblack T (erio T), added to an aqueous suspension of bovine retinal outer segments solubilized by digitonin, shows a light-induced absorption-increase at lambda = 645 nm. Erio T is shown to directly interact with miscellar metarhodopsin I and metarhodopsin II. The absorption-changes of erio T can be regarded as an indication of the transition from the metarhodopsin I conformation (with associated Ca2+) to the metarhodopsin II conformation (with associated H+).

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