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. 2004 Feb;48(2):681-4.
doi: 10.1128/AAC.48.2.681-684.2004.

Clonal multidrug-resistant Shigella dysenteriae type 1 strains associated with epidemic and sporadic dysenteries in eastern India

Gururaja Perumal Pazhani  1 , Bhaswati Sarkar, Thandavarayan Ramamurthy, S K Bhattacharya, Yoshifumi Takeda, S K Niyogi
Affiliations

Clonal multidrug-resistant Shigella dysenteriae type 1 strains associated with epidemic and sporadic dysenteries in eastern India

Gururaja Perumal Pazhani et al. Antimicrob Agents Chemother. 2004 Feb.

Abstract

Multidrug-resistant strains of Shigella dysenteriae type 1 were implicated in three outbreaks and sporadic cases of dysentery in eastern India in 2002 and 2003. After a hiatus of 14 years, this pathogen reemerged with an altered antibiotic resistance pattern. In addition to ampicillin, co-trimoxazole, tetracycline, chloramphenicol, and nalidixic acid, all the recent strains were resistant to norfloxacin, lomefloxacin, pefloxacin, and ofloxacin and showed reduced susceptibility to ciprofloxacin. Pulsed-field gel electrophoresis identified a new clone of S. dysenteriae type 1 that was associated with the recent outbreaks and sporadic cases. Based on the spatial and temporal spread of multidrug-resistant S. dysenteriae type 1, we predict that this clonal type may spread further in this region.

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Figures

FIG. 1.
FIG. 1.
PFGE profiles of S. dysenteriae type 1 strains after digestion with XbaI. Strains D1, D2, D44, 18, 21, and 25 represent the 2002 dysentery epidemic. Strain AZ11 was isolated from Aizal in 2003. Strains HU8, HU10, and HU29 were isolated during a 1988 outbreak in Tripura. BCH518 was isolated from a patient with a sporadic case in 1995 in Calcutta. Strains NK2217, NK2490, and NK2678 and strains NT4359, H14174, and H16576 were isolated from patients with sporadic cases of dysentery at BCRCH and IDH, respectively, in 2002. The molecular size marker (leftmost lane) used here is the PFG lambda ladder.
FIG. 2.
FIG. 2.
Dendrogram analysis of the S. dysenteriae type 1 strains based on the PFGE profiles with Diversity Database software (Bio-Rad) employing the UPGMA method, which grouped clusters A and B.
See this image and copyright information in PMC

References

    1. Dutta, P., D. Dutta, S. K. Bhattacharya, D. Sen, U. Mitra, A. R. Ghose, M. Lahiri, and B. C. Deb. 1989. Clinical and bacteriological profiles of shigellosis in Calcutta before and after an epidemic (1984-87). Indian J. Med. Res. 89:132-137. - PubMed
    1. Goma Epidemiology Group. 1995. Public health impact of Rwandan refugee crisis: what happened in Goma, Zaire, in July 1994? Lancet 345:339-344. - PubMed
    1. Guerin, P. J., C. Brasher, E. Baron, D. Mic, F. Grimont, M. Rayan, P. Aavitsland, and D. Legros. 2003. Shigella dysenteriae serotype 1 in West Africa: intervention strategy for an outbreak in Sierra Leone. Lancet 362:705-706. - PubMed
    1. Gunderson, K., and G. Chu. 1991. Pulsed-field electrophoresis of megabase-sized DNA. Mol. Cell. Biol. 11:3348-3354. - PMC - PubMed
    1. Iwalokun, B. A., G. O. Gbenle, S. I. Smith, A. Ogunledun, K. A. Akinsinde, and E. A. Omonigbehin. 2001. Epidemiology of shigellosis in Lagos, Nigeria: trends in antimicrobial resistance. J. Health Popul. Nutr. 19:183-190. - PubMed

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