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. 2004 Feb 1;10(3):455-7.
doi: 10.3748/wjg.v10.i3.455.

Probiotics inhibit TNF-alpha-induced interleukin-8 secretion of HT29 cells

Ai-Ping Bai  1 , Qin Ouyang, Wen Zhang, Chun-Hui Wang, Sheng-Fu Li
Affiliations

Probiotics inhibit TNF-alpha-induced interleukin-8 secretion of HT29 cells

Ai-Ping Bai et al. World J Gastroenterol. .

Abstract

Aim: To study the effect of probiotics on interleukin-8 secretion in intestinal epithelia when stimulated by proinflammatory cytokines.

Methods: Colonic adenocarcinoma HT29 cells were cultured and divided into four groups: control, TNF-alpha (group T in short), bifidobacterium (group B), lactobacillus (group L). B. Longum and L. bulgaricus were suspended in culture medium with a concentration of 1 x 10(8) cfu/ml and added into 24 wells respectively. One hour later TNF-alpha (10 ng/ml) was added into each well of groups T, B, L. The supernatants were collected and measured for IL-8 after 3 hours, nuclear factor-kappaB (NF-kappaB) p65 was also examined by Western blotting.

Results: There was less interleukin-8 secretion in HT29 cells when preincubated with B. Longum or L. bulgaricus compared with group T. Less p65 appeared in nuclei in groups B and L compared with group T, as detected by Western blot.

Conclusion: Probiotics can suppress interleukin-8 secretion in intestinal epithelia when stimulated by proinflammatory cytokines, which is most likely mediated by NF-kappaB.

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Figures

Figure 1
Figure 1
Concentrations of IL-8 in each group (mean ± SD). Natural interleukin-8 expression was seldom found in HT29 cells of control group. When stimulated by TNF-α (10 ng/ml), HT29 cells secreted a large number of IL-8, and the concentra-tions IL-8 in groups T, B and L were significantly increased than that in control (P < 0.001). However, there was some dif-ference in the concentration of IL-8 among groups T, B and L. There was less interleukin-8 secretion in HT29 cells when preincubated with B. Longum or L. bulgaricus in groups B and L compared with group T (P = 0.002, 0.01, respectively), but there was no difference in concentration of IL-8 between groups B and L (P = 0.068).
Figure 2
Figure 2
Expression of nuclear NF-κB p65 in each group. Line 1: group T, Line 2: control, Line 3: group L, Line 4: group B.
See this image and copyright information in PMC

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