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. 2004 Feb;186(4):1197-9.
doi: 10.1128/JB.186.4.1197-1199.2004.

PriA is essential for viability of the Escherichia coli topoisomerase IV parE10(Ts) mutant

Gianfranco Grompone  1 , Vladimir Bidnenko, S Dusko Ehrlich, Bénédicte Michel
Affiliations

PriA is essential for viability of the Escherichia coli topoisomerase IV parE10(Ts) mutant

Gianfranco Grompone et al. J Bacteriol. 2004 Feb.

Abstract

The parE10(Ts) mutation, which renders Escherichia coli thermosensitive for growth by inactivation of the essential E. coli topoisomerase topo IV, is lethal at all temperatures when PriA, the main replication restart protein, is absent. This lethality is suppressed by the activation of a PriA-independent replication restart pathway (dnaC809 mutation). This result suggests that topo IV acts prior to full-chromosome replication completion.

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