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. 1992 Dec;58(12):3868-72.
doi: 10.1128/aem.58.12.3868-3872.1992.

Monoclonal antibodies that detect live salmonellae

R Torensma  1 , M J Visser, C J Aarsman, M J Poppelier, R van Beurden, A C Fluit, J Verhoef
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Monoclonal antibodies that detect live salmonellae

R Torensma et al. Appl Environ Microbiol. 1992 Dec.

Abstract

Nine immunoglobulin G and nine immunoglobulin M murine monoclonal antibody-producing hybridomas reactive with live Salmonella bacteria were obtained from several fusions of immune spleen cells and Sp2/0 myeloma cells. The antibodies were selected by the magnetic immunoluminescence assay. The monoclonal antibodies were reactive with serogroups A, B, C1, C2, D, E, and K and Salmonella choleraesuis subsp. diarizonae. Each monoclonal antibody proved to be reactive with a distinct serotype. Clinical isolates belonging to these Salmonella serogroups could be detected. Reactivity with non-Salmonella bacteria proved to be minor.

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References

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