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. 2004 Feb 6;303(5659):832-5.
doi: 10.1126/science.1091266.

Genome-wide RNAi analysis of growth and viability in Drosophila cells

Michael Boutros  1 , Amy A Kiger, Susan Armknecht, Kim Kerr, Marc Hild, Britta Koch, Stefan A Haas, Renato Paro, Norbert Perrimon; Heidelberg Fly Array Consortium
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Genome-wide RNAi analysis of growth and viability in Drosophila cells

Michael Boutros et al. Science. .

Abstract

A crucial aim upon completion of whole genome sequences is the functional analysis of all predicted genes. We have applied a high-throughput RNA-interference (RNAi) screen of 19,470 double-stranded (ds) RNAs in cultured cells to characterize the function of nearly all (91%) predicted Drosophila genes in cell growth and viability. We found 438 dsRNAs that identified essential genes, among which 80% lacked mutant alleles. A quantitative assay of cell number was applied to identify genes of known and uncharacterized functions. In particular, we demonstrate a role for the homolog of a mammalian acute myeloid leukemia gene (AML1) in cell survival. Such a systematic screen for cell phenotypes, such as cell viability, can thus be effective in characterizing functionally related genes on a genome-wide scale.

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