Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2004 Feb;42(2):822-4.
doi: 10.1128/JCM.42.2.822-824.2004.

Molecular characterization of resistance to mupirocin in methicillin-susceptible and -resistant isolates of Staphylococcus aureus from nasal samples

Fernando Chaves  1 , Jesus García-Martínez, Sonia de Miguel, Joaquín R Otero
Affiliations

Molecular characterization of resistance to mupirocin in methicillin-susceptible and -resistant isolates of Staphylococcus aureus from nasal samples

Fernando Chaves et al. J Clin Microbiol. 2004 Feb.

Abstract

A total of 15 of 101 (14.8%) nasal methicillin-resistant Staphylococcus aureus (MRSA) isolates exhibited mupirocin resistance (Mup(r)) compared with 1 of 154 (0.6%) methicillin-susceptible Staphylococcus aureus isolates. A total of 14 (93%) isolates exhibiting high-level Mup(r) belonged to a single clone. Horizontal plasmid transfer and transmission of Mup(r) strains contribute to a high incidence of Mup(r) MRSA at our institution.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
PFGE DNA patterns of Mupr S. aureus isolates from nasal samples. Lanes 1 to 7 represent genotypes A1 (isolate 1), A2 (isolate 3), A3 (isolate 4), A4 (isolate 2), A5 (isolate 6), B (isolate 7), and C (isolate 13), respectively. Lanes 8 to 11 represent the banding patterns of paired Mups and subsequently recovered Mupr S. aureus isolates (isolates 5, 12, 14, and 15) from nasal samples of four patients.
FIG. 2.
FIG. 2.
Southern blot of HindIII-digested plasmid DNA from selected Mupr S. aureus isolates hybridized with an ileS2 probe. Lane 1, MRSA isolate 1 (Hi-Mupr); lane 2, MRSA isolate 4 (low-level Mupr); lane 3, MRSA isolate 5 (Hi-Mupr); lane 4, MRSA isolate 8 (Hi-Mupr); lane 5, MSSA isolate 13 (Hi-Mupr).
See this image and copyright information in PMC

References

    1. Anthony, R. M., A. M. Connor, E. G. M. Power, and G. L. French. 1999. Use of the polymerase chain reaction for rapid detection of high-level mupirocin resistance in staphylococci. Eur. J. Clin. Microbiol. Infect. Dis. 18:30-34. - PubMed
    1. Boyce, J. M. 1996. Preventing staphylococcal infections by eradicating nasal carriage of Staphylococcus aureus: proceeding with caution. Infect. Control Hosp. Epidemiol. 17:775-779. - PubMed
    1. Cookson, B. D. 1998. The emergence of mupirocin resistance: a challenge to infection control and antibiotic prescribing practice. J. Antimicrob. Chemother. 41:11-18. - PubMed
    1. Dominguez, M. A., H. De Lencastre, J. Liñares, and A. Tomasz. 1994. Spread and maintenance of a dominant methicillin-resistant Staphylococcus aureus (MRSA) clone during an outbreak of MRSA disease in a Spanish hospital. J. Clin. Microbiol. 32:2081-2087. - PMC - PubMed
    1. Fuchs, P. C., R. N. Jones, and A. L. Barry. 1990. Interpretive criteria for disk diffusion susceptibility testing of mupirocin, a topical antibiotic. J. Clin. Microbiol. 28:608-609. - PMC - PubMed

MeSH terms