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Comparative Study
. 1992 Oct;13(4):540-3.

Nonradioactive southwestern analysis using chemiluminescent detection

S Dooley  1 , C Welter, N Blin
Affiliations
  • PMID: 1476722
Comparative Study

Nonradioactive southwestern analysis using chemiluminescent detection

S Dooley et al. Biotechniques. 1992 Oct.

Abstract

Replacing radioactively labeled probes by nonradioactive ones and detection by chemiluminescence instead of colorimetry allows a nonhazardous handling and offers the possibility of easily reprobing filters in Southwestern analysis. Using the described procedure, we were able to determine the molecular weight of DNA-binding proteins and achieve a high signal-to-noise ratio.

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