doi: 10.1101/gr.2.2.144.
A lock-docking oligo(dT) primer for 5' and 3' RACE PCR
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- PMID: 1477669
- DOI: 10.1101/gr.2.2.144
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A lock-docking oligo(dT) primer for 5' and 3' RACE PCR
PCR Methods Appl.
1992 Nov.
Free article
Abstract
We describe a method that can be used to obtain and sequence 3' and 5' ends of cDNA transcripts directly from PCR products. The method employs a modified oligo(dT) primer that enables it to "lock-dock" at the junction of gene-specific cDNA sequence and a natural (3') or appended (5') poly(A) tail. As a result, discrete, first-round PCR products are obtained that are easily isolated and sequenced directly.
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