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. 1992 Nov;120(2):79-85.
doi: 10.1007/BF00578292.

The solid phase attachment of fungal hyphae in an ELISA to screen for antifungal antibodies

J N Banks  1 , S J Cox
Affiliations

The solid phase attachment of fungal hyphae in an ELISA to screen for antifungal antibodies

J N Banks et al. Mycopathologia. 1992 Nov.

Abstract

A method to immobilize fungal hyphae onto the wells of 96-well microplates for use in an in-direct ELISA to screen for antifungal antibodies in sera and cell culture supernatants is described. The hyphae from three genera (Penicillium, Eurotium and Fusarium) were successfully attached by overnight drying onto wells precoated with poly-L-lysine and glutaraldehyde. Microscopy revealed that the hyphae remained attached to the wells throughout the ELISA and antiserum titrations showed that the attached hyphae were uniformly coated and remained reactive. Background absorbances were low and the plates could be stored at -20 degrees C without loss of reactivity.

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References

    1. J Immunol Methods. 1985 Jan 21;76(1):63-72 - PubMed
    1. J Immunol Methods. 1984 Oct 26;73(2):427-35 - PubMed
    1. J Virol Methods. 1984 Feb;8(1-2):87-98 - PubMed
    1. Clin Chim Acta. 1976 Jul 1;70(1):1-31 - PubMed
    1. Methods Enzymol. 1972;25:623-51 - PubMed

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