doi: 10.1042/cs0520229.
Enzyme activities in human liver biopsies: assay methods and activities of some lysosomal and membrane-bound enzymes in control tissue and serum
- PMID: 14804
- DOI: 10.1042/cs0520229
Item in Clipboard
Enzyme activities in human liver biopsies: assay methods and activities of some lysosomal and membrane-bound enzymes in control tissue and serum
Clin Sci Mol Med.
1977 Mar.
Abstract
1. Highly sensitive technique are described for the assay of plasma membrane (5'-nucleotidase, alkaline phosphatase), microsomal (neutral alpha-glucosidase, leucyl-2-naphthylamidase) and biliary canalicular (gamma-glutamyltransferase) enzymes and for nine acid hydrolases (acid phosphatase, phosphodiesterase, beta-glucosidase, alpha-glucosidase, alpha-galactosidase, beta-galactosidase, alpha-mannosidase, N-acetyl-beta-glucosaminidase, beta-glucuronidase) in human liver. 2. Optimum and specific assay systems have been developed which give linear kinetics for all enzymes. 3. The range of enzyme activities in samples of human liver, obtained by closed needle biopsy, and sera have been determined.
Similar articles
-
Enzymatic activities in jejunal biopsy specimens from patients with the stagnant-loop syndrome.Scand J Gastroenterol. 1983 Jul;18(5):599-602. doi: 10.3109/00365528309181644. Scand J Gastroenterol. 1983. PMID: 6144177
-
Subcellular fractionation studies on peroral jejunal biopsies from the dog.Res Vet Sci. 1978 Jul;25(1):94-100. Res Vet Sci. 1978. PMID: 30125
-
Analytical subcellular fractionation of needle-biopsy specimens from human liver.Biochem J. 1978 Aug 15;174(2):435-46. doi: 10.1042/bj1740435. Biochem J. 1978. PMID: 708396 Free PMC article.
-
Effect of bile-duct ligation on organelle marker enzymes in the liver and serum of rats.Clin Sci Mol Med. 1975 Apr;48(4):307-13. doi: 10.1042/cs0480307. Clin Sci Mol Med. 1975. PMID: 236111
-
The cellular processing of lysosomal enzymes and related proteins.Rev Physiol Biochem Pharmacol. 1980;87:169-210. doi: 10.1007/BFb0030898. Rev Physiol Biochem Pharmacol. 1980. PMID: 6999583 Review. No abstract available.
Cited by
-
Enhancement of ileal adaptation by prednisolone after proximal small bowel resection in the rat.Gut. 1979 Oct;20(10):858-64. doi: 10.1136/gut.20.10.858. Gut. 1979. PMID: 533697 Free PMC article.
-
Studies on the kinetic properties and subcellular localization of adenine nucleotide phosphatases in peripheral blood lymphocytes from control subjects and patients with common variable primary hypogammaglobulinaemia.Clin Exp Immunol. 1982 Aug;49(2):393-400. Clin Exp Immunol. 1982. PMID: 6127180 Free PMC article.
-
Enzymic oxidation of unconjugated bilirubin by rat liver.Biochem J. 1986 Jun 15;236(3):625-33. doi: 10.1042/bj2360625. Biochem J. 1986. PMID: 3790083 Free PMC article.
-
Electron microscopic cytochemical localization of nucleoside phosphatases in normal and chronic granulocytic leukaemic human neutrophils.Histochem J. 1981 Jan;13(1):73-84. doi: 10.1007/BF01005841. Histochem J. 1981. PMID: 6112213
-
Glucagon-stimulable adenylyl cyclase in rat liver. The impact of streptozotocin-induced diabetes mellitus.J Clin Invest. 1984 Apr;73(4):1013-23. doi: 10.1172/JCI111286. J Clin Invest. 1984. PMID: 6323532 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials