Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1950 Nov;34(2):147-59.
doi: 10.1085/jgp.34.2.147.

Methyl green. III. Reaction with desoxyribonucleic acid, stoichiometry, and behavior of the reaction product

N B KURNICK, M FOSTER

Methyl green. III. Reaction with desoxyribonucleic acid, stoichiometry, and behavior of the reaction product

N B KURNICK et al. J Gen Physiol. 1950 Nov.

Abstract

1. Methyl green ("ethyl green") C. I. Number 685 was examined and found to behave identically with methyl green C. I. Number 684 (no longer available) in respect to molar extinction coefficient, effect of combination with polymerized DNA, failure to react with depolymerized DNA, and effect of pH. 2. The mass law permits the calculation of P/dye. This is found to be 13 P/dye. The same value is obtained when an excess of methyl green is caused to fade by adjusting the pH to 7.5. 3. The compound formed by methyl green with DNA has the same maximum absorption at 642.5 to 645 mmicro in the pH range 3.5-7.8, whereas the free dye fades markedly above pH 5.0.

PubMed Disclaimer

References

    1. J Gen Physiol. 1950 Jan 20;33(3):243-64, pl - PubMed
    1. J Gen Physiol. 1950 Mar;33(4):363-77 - PubMed
    1. J Gen Physiol. 1950 Jan 20;33(3):265-74 - PubMed