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Comparative Study
. 1992 Dec;64(2):145-8.
doi: 10.1016/0165-4608(92)90345-9.

Fluorescence in situ hybridization to determine engraftment status after murine bone marrow transplant

A L Hawkins  1 , R J Jones, B A Zehnbauer, M S Zicha, M J Collector, S J Sharkis, C A Griffin
Affiliations
Comparative Study

Fluorescence in situ hybridization to determine engraftment status after murine bone marrow transplant

A L Hawkins et al. Cancer Genet Cytogenet. 1992 Dec.

Abstract

The mouse Y-specific DNA sequence pY2 was used as a probe for fluorescence in situ hybridization (FISH) to evaluate murine hematopoietic tissues after sex-mismatched bone marrow transplant (BMT). The pY2 probe was localized to the long arm of the Y chromosome on BM metaphases. Hybridization of pY2 in FISH of interphase cells from BM, spleen, and thymus after BMT was compared with Southern blot analysis; both methods gave comparable results. Only FISH was able to analyze post-BMT peripheral blood (PB) samples successfully, and provides a useful method for following engraftment status in the mouse on an ongoing basis.

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