Cell membranes and multilamellar vesicles: influence of pH on solvent induced damage

Abstract

Pigment leakage from sheep and horse erythrocytes and from red beet tissue induced by non-polar solvents was determined as a function of pH. The results were compared to disruption of multilamellar vesicles (MLV) composed of phospholipids with equimolar cholesterol under identical conditions of solvent exposure and pH. Solvent access to cholesterol was used to measure vesicle disruption. MLV were made from 1,2-dioleoyl phosphatidylethanolamine, sphingomyelin (SP) and various phosphatidylcholines to simulate the major lipid components of membranes. Pigment leakage from erythrocytes caused by petroleum hydrocarbon (b.p. 60-80 degrees C) was maximal at pH 2-4 and at pH 10, but minimal at pH 6.8; alcohols caused less pigment leakage than petroleum hydrocarbon. Beta-cyanin leakage from beet tissue induced by petroleum hydrocarbon was maximal at pH 2, with very little leakage at pH 4, 6.6 and pH 10. Alcohols caused minimal damage to beet tissue above pH 2. Cholesterol removal by petroleum hydrocarbon from MLV of mixed lipid composition was maximal at pH 2-4, reduced at pH 6.8 and minimal at pH 10. Lipid mixtures in which fatty acyl side chains of one phospholipid were of a different length than the other lost more sterol than mixtures in which the acyl side chains were of identical chain length. MLV with more than 25% SP lost more sterol than those with less or no SP. Results show that in mixtures of phospholipids, SP exposes the hydrocarbon phase of a bilayer to solvent extraction, a property that was also observed in native membranes.(ABSTRACT TRUNCATED AT 250 WORDS)