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. 1992 Nov:29 ( Pt 6):663-7.
doi: 10.1177/000456329202900608.

Improved flow cytometric method for HLA-B27 typing

W C Janssen  1 , J A Rouwen, J J Hoffmann
Affiliations

Improved flow cytometric method for HLA-B27 typing

W C Janssen et al. Ann Clin Biochem. 1992 Nov.

Abstract

HLA-B27 is a cell marker of clinical interest because of its high association with certain diseases. The HLA-B27 antigen was detected on lymphocytes using a monoclonal antibody in an indirect immuno-fluorescence assay using a fluorescence flow cytometer. The considerable crossreaction of the monoclonal antibody with the HLA-B7 antigen was effectively suppressed by masking it by means of human anti-HLA-B7 antiserum. The flow cytometric method was evaluated by comparing the results with those obtained by the standard lymphocytotoxicity test and showed complete agreement in 107 selected patient samples.

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