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Comparative Study
. 1992;37(5):353-6.
doi: 10.1007/BF02815661.

Isolation of a new restriction enzyme, ApaCI, an isoschizomer of BamHI produced by Acetobacter pasteurianus

J Grones  1 , J Turna
Affiliations
Comparative Study

Isolation of a new restriction enzyme, ApaCI, an isoschizomer of BamHI produced by Acetobacter pasteurianus

J Grones et al. Folia Microbiol (Praha). 1992.

Abstract

A new Type II restriction endonuclease ApaCI purified from Acetobacter pasteurianus is an isoschizomer of BamHI that cleaves at the nucleotide sequence 5'-G/GATCC-3' of double-stranded DNA. The single restriction activity present in this strain permits rapidly purified 30,000 units of cleavage activity from 10 g of freshly harvested cells. The resulting ApaCI preparation is free of contaminant nuclease activities that might interfere with in vitro manipulation of DNA.

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References

    1. Methods Enzymol. 1983;101:20-78 - PubMed
    1. Nucleic Acids Res. 1988 Mar 25;16(6):2718 - PubMed
    1. J Mol Biol. 1975 Jan 25;91(3):315-28 - PubMed
    1. Nucleic Acids Res. 1983 Jul 11;11(13):4409-15 - PubMed
    1. Nucleic Acids Res. 1982 Oct 11;10(19):5747-52 - PubMed

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