Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1992;37(5):353-6.
doi: 10.1007/BF02815661.

Isolation of a new restriction enzyme, ApaCI, an isoschizomer of BamHI produced by Acetobacter pasteurianus

J Grones  1 , J Turna
Affiliations
Comparative Study

Isolation of a new restriction enzyme, ApaCI, an isoschizomer of BamHI produced by Acetobacter pasteurianus

J Grones et al. Folia Microbiol (Praha). 1992.

Abstract

A new Type II restriction endonuclease ApaCI purified from Acetobacter pasteurianus is an isoschizomer of BamHI that cleaves at the nucleotide sequence 5'-G/GATCC-3' of double-stranded DNA. The single restriction activity present in this strain permits rapidly purified 30,000 units of cleavage activity from 10 g of freshly harvested cells. The resulting ApaCI preparation is free of contaminant nuclease activities that might interfere with in vitro manipulation of DNA.

PubMed Disclaimer

References

    1. Methods Enzymol. 1983;101:20-78 - PubMed
    1. Nucleic Acids Res. 1988 Mar 25;16(6):2718 - PubMed
    1. J Mol Biol. 1975 Jan 25;91(3):315-28 - PubMed
    1. Nucleic Acids Res. 1983 Jul 11;11(13):4409-15 - PubMed
    1. Nucleic Acids Res. 1982 Oct 11;10(19):5747-52 - PubMed

Publication types

MeSH terms

LinkOut - more resources