Amplicon: software for designing PCR primers on aligned DNA sequences
- PMID: 14962918
- DOI: 10.1093/bioinformatics/bth121
Amplicon: software for designing PCR primers on aligned DNA sequences
Abstract
Summary: Amplicon is a program for designing PCR primers on aligned groups of DNA sequences. The most important application for Amplicon is the design of 'group-specific' PCR primer sets that amplify a DNA region from a given taxonomic group but do not amplify orthologous regions from other taxonomic groups.
Availability: Amplicon is freely available as a script that will run on any platform with Python 2.3 installed (http://www.python.org). It is also available as a Windows executable. Free downloads that do not require registration can be found at http://www.aad.gov.au/amplicon
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