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. 2004 Mar;56(3):431-4.
doi: 10.1016/j.mimet.2003.11.015.

A rapid DNA extraction method for PCR identification of fungal indoor air contaminants

Timothy R Dean  1 , Doris Betancourt, Marc Y Menetrez
Affiliations

A rapid DNA extraction method for PCR identification of fungal indoor air contaminants

Timothy R Dean et al. J Microbiol Methods. 2004 Mar.

Abstract

Following air sampling fungal DNA needs to be extracted and purified to a state suitable for laboratory use. Our laboratory has developed a simple method of extraction and purification of fungal DNA appropriate for enzymatic manipulation and Polymerase Chain Reaction (PCR) applications. The methodology described is both rapid and cost effective for use with multiple fungal organisms.

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