Potassium phosphate induces systemic protection in barley to powdery mildew infection

Abstract

In laboratory tests, treatment of the first leaves of barley (Hordeum vulgare L cv Golden Promise) with potassium phosphate led to significant reduction in infection of the second leaves with the powdery mildew fungus Blumeria graminis f sp hordei Marchal, with a 25 mM treatment giving 89% reduction in infection. Although the optimal interval between phosphate treatment of the first leaves and mildew inoculation of the second leaves was 2 days, significant protection was still obtained if the interval was increased to 12 days. Protection against powdery mildew infection was not as effective when the potassium phosphate was applied as a seed treatment or root drench. Phosphate treatment of the first leaves led to significant increases in activities of phenylalanine ammonia lyase (PAL), peroxidase and lipoxygenase in second leaves. Enzyme activities, especially PAL and peroxidase, were increased further when second leaves of phosphate-treated plants were inoculated with powdery mildew. Phosphate treatment of the first leaves did not adversely affect plant growth and, in a field trial, 25 mM potassium phosphate provided 70% control of mildew and gave a small increase in grain yield.