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. 1992 Jul 31;186(2):1037-41.
doi: 10.1016/0006-291x(92)90851-b.

Incorporation into planar lipid bilayers of ATP-regulated K+ channels from membranes of the insulin secreting beta-cell line, HIT T15

Y Oosawa  1
Affiliations

Incorporation into planar lipid bilayers of ATP-regulated K+ channels from membranes of the insulin secreting beta-cell line, HIT T15

Y Oosawa. Biochem Biophys Res Commun. .

Abstract

Ion channels were incorporated into planar lipid bilayers following fusion of vesicles from the membrane of an insulin-secreting beta-cell line, HIT T15. The channel was completely blocked by 0.5 mM ATP. The channel retained the same ATP-dependence, voltage-sensitivity and single channel conductance as the ATP-regulated K+ channel that found in isolated membrane patches.

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