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. 2004 Jan;68(1):71-5.

Computer-based analysis of Haemophilus parasuis protein fingerprints

Simone Oliveira  1 , Carlos Pijoan
Affiliations

Computer-based analysis of Haemophilus parasuis protein fingerprints

Simone Oliveira et al. Can J Vet Res. 2004 Jan.

Abstract
in English, French

The present study aimed to compare the whole-cell protein profiles of Haemophilus parasuis field isolates by using a computer-based analysis, and evaluate the relationship between polyacrylamide gel electrophoresis (PAGE) type and virulence potential based on isolation site. A dendrogram clustering isolates with similar protein profiles was generated. Haemophilus parasuis isolates were grouped into 2 major PAGE type groups. The PAGE type II isolates were characterized by the presence of major proteins with molecular weights varying from between 36 and 38 kDa and included 90.7% of the isolates recovered from systemic sites, such as pleura, pericardium, peritoneum, lymph nodes, joints, and brain. Isolates classified as PAGE type I were characterized by the absence of this group of proteins and included 83.4% of the isolates recovered from the upper respiratory tract of healthy animals. The present study further corroborates the existence of a unique group of major proteins in potentially virulent H. parasuis isolates.

Dans cette étude, une comparaison a été effectuée entre les profils de protéines des cellules entières d’isolats cliniques de Haemophilus parasuis par analyse informatique, ainsi que d’évaluer la relation entre les patrons électrophorétiques obtenus sur gel de polyacrylamide (PAGE) et le potentiel de virulence basé sur le site d’isolement. Un dendrogramme regroupant les isolats ayant des profils de protéines similaires a été produit. Les isolats d’H. parasuis ont été regroupés en 2 groupes majeurs basés sur leur profil PAGE. Les isolats appartenant au profil de type II étaient caractérisés par la présence de protéines majeures avec des poids moléculaires variant entre 36 et 38 kDa et comptait 90,7 % des isolats retrouvés des sites d’infection systémique tels que la plèvre, le péricarde, le péritoine, les nœuds lymphatiques, les articulations et le cerveau. Les isolats classées comme appartenant au profil de type I étaient caractérisés par l’absence de ce groupe de protéines et comprenait 83,5 % des isolats provenant du tractus respiratoire supérieur d’animaux en santé. Cette étude corrobore l’existence d’un groupe unique de protéines majeures parmi les isolats potentiellement virulents de H. parasuis.

(Traduit par Docteur Serge Messier)

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Figures

Figure 1
Figure 1
Cluster analysis of Haemophilus parasuis field isolates based on whole-cell protein profiles (sodium dodecyl sulfate polyacrylamide gel electrophoresis [SDS-PAGE], Pearson correlation, UPMGA). The scale indicates the percent of similarity. The columns in the figure indicate the isolate identification, site of isolation, serotype and genotype groups, as defined by Oliveira et al (10). Relevant clusters of strains are shown by gray lines. Clusters were defined based on the calculation of the point-bisectional correlation (BioNumerics).
Figure 2
Figure 2
Distribution of Haemophilus parasuis field isolates according to polyacrylamide gel electrophoresis (PAGE) type and site of isolation.
See this image and copyright information in PMC

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