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. 2004 Mar;48(3):1004-6.
doi: 10.1128/AAC.48.3.1004-1006.2004.

Evaluation of acute hematologic and long-term pulmonary toxicities of radioimmunotherapy of Cryptococcus neoformans infection in murine models

E Dadachova  1 , R A Bryan, A Frenkel, T Zhang, C Apostolidis, J S Nosanchuk, J D Nosanchuk, A Casadevall
Affiliations

Evaluation of acute hematologic and long-term pulmonary toxicities of radioimmunotherapy of Cryptococcus neoformans infection in murine models

E Dadachova et al. Antimicrob Agents Chemother. 2004 Mar.

Abstract

We evaluated acute hematological and long-term pulmonary toxicity of radioimmunotherapy in murine models of Cryptococcus neoformans infection. Activities up to 250 microCi were well tolerated by healthy A/JCr mice for (213)Bi-18B7 and (188)Re-18B7 monoclonal antibodies. In infected mice, doses up to 150 microCi produced only transient toxicity. The lungs of treated mice had no evidence of radiation fibrosis.

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Figures

FIG. 1.
FIG. 1.
Platelet counts in A/JCr mice. Healthy mice (a and c) and C. neoformans-infected mice (b and d) received various doses of 213Bi-18B7 (a and b) or 188Re-18B7 (c and d). A “0” indicates infected nontreated mice. C. neoformans-infected mice treated with 200 and 250 μCi of 213Bi-18B7 (b) died by day 7 posttreatment. The platelet count in healthy nontreated A/JCr mice was (8.8 ± 2.4) × 108 platelets/ml.
FIG. 2.
FIG. 2.
Micrographs of hematoxylin-and-eosin-stained lungs from BALB/c mice infected IT with C. neoformans and treated with radiolabeled antibodies. Mice were sacrificed 5 months after RIT. (a) Infected control for Bi group (no RIT); (b) 200 μCi of 213Bi-18B7; (c) infected control for Re group (no RIT); (c) 200 μCi of 188Re-18B7.
See this image and copyright information in PMC

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