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. 2004 Mar;48(3):1058-60.
doi: 10.1128/AAC.48.3.1058-1060.2004.

Inhibition of beta-lactamase II of Bacillus cereus by penamaldic derivatives of penicillins

Pilar Gutiérrez Navarro  1 , Bartolomé Quintero Osso, Raquel García Ortiz, Pedro J Martínez De Las Parras, María I Martínez Puentedura, M Carmen Cabeza González
Affiliations

Inhibition of beta-lactamase II of Bacillus cereus by penamaldic derivatives of penicillins

Pilar Gutiérrez Navarro et al. Antimicrob Agents Chemother. 2004 Mar.

Abstract

The penamaldic derivatives of amoxicillin, ampicillin, and penicillins G and V, stabilized with Zn(2+), were obtained from a methanolic medium. The enzymatic kinetic results show that the these derivatives elicit reversible inhibition of the enzyme metallo-beta-lactamase from Bacillus cereus, with inhibition constant values determined at pH 7.0 and 25 degrees C.

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Figures

FIG. 1.
FIG. 1.
Scheme of the penamaldic derivatives.
FIG. 2.
FIG. 2.
Plot of the data according to equation 2 for the calculation of KI following the entire course of the reaction. Eight microliters of enzyme solution (equivalent to 0.176 mg of the commercial sample) was used. The substrate concentration was 5 × 10−4 M, and the total volume was 0.8 ml.
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References

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