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. 2004 Mar 19;315(4):845-9.
doi: 10.1016/j.bbrc.2004.01.142.

Amplification of nuclear aldolase A in mouse cell mutants resistant to Hoechst 33342

Wataru Satou  1 , Hiroshi Tanimoto, Ryo Ukekawa, Michihiko Fujii, Dai Ayusawa
Affiliations

Amplification of nuclear aldolase A in mouse cell mutants resistant to Hoechst 33342

Wataru Satou et al. Biochem Biophys Res Commun. .

Abstract

5-Bromodeoxyuridine (BrdU) induces a phenomenon similar to cellular senescence in mammalian cells. AT-binding ligands such as Hoechst 33258 synergistically potentiate the effect of BrdU. We isolated mouse FM3A cell mutants resistant to Hoechst 33342 and characterized two highly resistant mutants. Two-dimensional gel electrophoresis followed by peptides sequence tags revealed that nuclear aldolase A was markedly increased in both mutants. Western blot analysis confirmed that nuclear aldolase A was increased leaving cytosolic aldolase A unaltered. Its mRNA levels were also increased in the mutants. Enforced expression of aldolase A conferred resistance to Hoechst 33342 on wild-type cells. Taken together, nuclear aldolase A was shown to somehow protect the cytotoxic effect of Hoechst 33342.

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