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. 2004 Mar 19;315(4):959-63.
doi: 10.1016/j.bbrc.2004.01.152.

Trichostatin A activates the osteopontin gene promoter through AP1 site

Ryosuke Sakata  1 , Shinji Minami, Yoshihiro Sowa, Munehito Yoshida, Tetsuya Tamaki
Affiliations

Trichostatin A activates the osteopontin gene promoter through AP1 site

Ryosuke Sakata et al. Biochem Biophys Res Commun. .

Abstract

In this study, we investigated osteoblastic differentiation by trichostatin A (TSA), a histone deacetylase inhibitor in mouse undifferentiated mesenchymal cell line. TSA increased the osteopontin (OPN) mRNA level and OPN protein. Deletion analysis of the promoter region revealed TSA-induced luciferase response was regulated by -75 to -65 of the OPN promoter. There was an AP1-binding sequence at the site of the OPN promoter. In an electrophoretic mobility shift assay, bands of the complexes were supershifted by addition of antibody to c-fos and phosphorylated c-jun. These data suggested that AP1 plays a crucial role in the TSA-induced OPN expression.

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