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. 2004 Mar;42(3):1302-4.
doi: 10.1128/JCM.42.3.1302-1304.2004.

Stability of polymorphic GC-rich repeat sequence-containing regions of Mycobacterium tuberculosis

Madalene Richardson  1 , Gian D van der Spuy, Samantha L Sampson, Nulda Beyers, Paul D van Helden, Robin M Warren
Affiliations

Stability of polymorphic GC-rich repeat sequence-containing regions of Mycobacterium tuberculosis

Madalene Richardson et al. J Clin Microbiol. 2004 Mar.

Abstract

Mycobacterium tuberculosis cultures were subjected to DNA fingerprinting with IS6110- and polymorphic GC-rich sequence (PGRS)-containing probes. The PGRS banding patterns remained highly stable during multiple cultures of specimens from one disease episode (0.5% changed) and during transmission in patients with close contact (1.9% changed). Characteristic PGRS-restriction fragment length polymorphism motifs for different strain groupings may indicate distant evolutionary events leading to the differentiation of M. tuberculosis strain lineages.

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Figures

FIG. 1.
FIG. 1.
PGRS banding patterns of strain sets (A to C) with changes. Arrows indicate either an additional band (sets A and B) or a band shift (set B). Characteristic banding-pattern motifs for different strain sets can be observed. Molecular sizes are indicated (in base pairs).
See this image and copyright information in PMC

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