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. 2004 Mar;42(3):1360-2.
doi: 10.1128/JCM.42.3.1360-1362.2004.

Development of a diagnostic PCR assay targeting the Mn-dependent superoxide dismutase gene (sodA) for identification of Streptococcus gallolyticus

Eiki Sasaki  1 , Ro Osawa, Yosuke Nishitani, Robert A Whiley
Affiliations

Development of a diagnostic PCR assay targeting the Mn-dependent superoxide dismutase gene (sodA) for identification of Streptococcus gallolyticus

Eiki Sasaki et al. J Clin Microbiol. 2004 Mar.

Abstract

A PCR-based assay to identify Streptococcus gallolyticus has been developed. The assay uses an oligonucleotide primer pair targeting a partial sequence of the manganese-dependent superoxide dismutase gene (sodA). The assay distinguished members of the S. gallolyticus group from other, closely related taxa successfully by yielding a 408-bp specific amplicon.

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Figures

FIG. 1.
FIG. 1.
Alignments of the sodA gene DNA sequences used in the present study and retrieved from the GenBank database (S. gallolyticus CIP105428T [GenBank accession no. AJ297183], S. bovis CIP102302 [GenBank accession no. Z95896], and S. equinus 102504T [GenBank accession no. Z95903]). Boldface letters indicate the positions of the primers in the sequences. The hyphens indicate the same bases as shown in S. gallolyticus CIP102302.
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