Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Aug;30(8):2173-6.
doi: 10.1128/jcm.30.8.2173-2176.1992.

Characterization of a DNA probe for detection of Mycobacterium tuberculosis complex in clinical samples by polymerase chain reaction

M Altamirano  1 , M T Kelly, A Wong, E T Bessuille, W A Black, J A Smith
Affiliations

Characterization of a DNA probe for detection of Mycobacterium tuberculosis complex in clinical samples by polymerase chain reaction

M Altamirano et al. J Clin Microbiol. 1992 Aug.

Abstract

We cloned and sequenced a DNA fragment from Mycobacterium tuberculosis for use in the identification of members of the M. tuberculosis complex. The DNA probe for culture confirmation had a sensitivity and a specificity of 100%. By using primers developed from this probe, the polymerase chain reaction detected 20 mycobacteria by ethidium bromide staining. This polymerase chain reaction system demonstrated 98% sensitivity and 100% specificity for detection of the M. tuberculosis complex in 200 sputum specimens.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Anal Biochem. 1979 Nov 15;100(1):188-97 - PubMed
    1. Tubercle. 1988 Mar;69(1):27-36 - PubMed
    1. Neurology. 1990 Oct;40(10):1617-8 - PubMed
    1. J Clin Microbiol. 1990 Nov;28(11):2437-41 - PubMed
    1. Lancet. 1989 Nov 4;2(8671):1069-71 - PubMed

Publication types

MeSH terms

LinkOut - more resources